![]() Last, you will incubate the bacterial culture with the antibiotic compound to quantify the effect of the compound on bacterial growth. You will learn how to calculate the colony forming units per milliliter (cfu/mL) of your bacterial culture using a cfu calculator. In a real lab, this experiment would take a week or more to complete, but in this simulation it only takes 30 minutes! Plate out countless agar plates in order to quantify the colonies from your serial dilutions, but don’t worry, not a single plate will be wasted. Make sure you use sterile techniques to avoid contaminating your bacterial cultures! One, will be ready to answer your questions and guide you through the steps needed to perform the serial dilution. ![]() You will learn much more by trial and error, and if you are up for the challenge you will be rewarded with some extra questions. Inquiry-based approach to quantify bacteriaįigure out how to quantify the bacterial cells by inquiry-based learning. The diluted inoculum is then added to a molten agar. ![]() But, you will only be able to succeed if you manage to combine smart experimental design, some simple mathematics, and proper microbial culture techniques. The pour plate technique requires a serial dilution of the mixed culture by means of a loop or pipette. Your mission is to quantify the effect of this antibiotic compound on bacterial growth. ![]() Your colleague has just returned from an expedition to the Amazon rainforest, and has brought back a promising sample a fungus that seems to inhibit bacterial growth. Did you know that it’s possible to count bacteria with your naked eye? In this simulation, you will learn how to quantify bacteria using the process of serial dilution. ![]()
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